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Inhibition by 5‐(tetradecyloxy)‐2‐furoic acid of fatty acid and cholesterol synthesis in isolated rat hepatocytes
Author(s) -
Panek Edwige,
Cook George A.,
Cornell Neal W.
Publication year - 1977
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533270
Subject(s) - nad+ kinase , fatty acid , chemistry , clinical chemistry , biochemistry , lipidology , ethanol , fatty acid synthesis , clofibrate , potency , cholesterol , in vitro , enzyme
Fatty acid and cholesterol synthesis in isolated rat hepatocytes were strongly inhibited by 5‐(tetradecyloxy)‐2‐furoic acid. With either 3 H 2 O or [2‐ 14 C]acetate as the labeled precursor, the concentrations of inhibitor causing 50% decrease in fatty acid and cholesterol synthesis were, respectively, <0.005 mM and 0.020 mM. At 0.1 mM inhibitor, citrate concentration in cells from fed rats was increased by 75%; lactate and pyruvate concentrations were decreased by 30%; ethanol oxidation was decreased by 20%; with cells from starved rats, the mitochondrial [NAD + ]/[NADH] was decreased. Other parameters were unaffected. Both its potency and its specificity indicate that 5‐(tetradecyloxy)‐2‐furoic acid will be useful in studies on the regulation of lipid biosynthesis.