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Determination of peroxide value by the colorimetric iodine method with protection of iodide as cadmium complex
Author(s) -
Takagi Toru,
Mitsuno Yuichiro,
Masumura Mayumi
Publication year - 1978
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533257
Subject(s) - iodide , iodine , chemistry , absorbance , cadmium , solvent , chloroform , colorimetry , peroxide , hydrogen peroxide , iodine value , inorganic chemistry , chromatography , organic chemistry
Abstract Improved procedures of the Swoboda and Lea method for the determination of peroxide values (POV) of fats and lipids are presented. After oxidation of iodide to iodine with the sample for 5 min under an inert atmosphere, an excess of the iodide ion is immediately converted to cadmium complex for protection from atmospheric oxygen. The iodine is measured colorimetrically at 358 or 410 nm, and POV is calculated from the absorbance. This method permits the rapid determination of POV with a small amount of sample at a moderate cost using usual glasswares. For the analysis of lipids in biological materials or food products, the chloroform solution obtained by the Bligh and Dyer method is directly subjected to this procedure without evaporation of the solvent. Conversions between POV obtained by the different methods are discussed.