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Effect of double bond position in octadecenoates upon hydrolysis by pancreatic lipase
Author(s) -
Heimermann Wayne H.,
Holman Ralph T.,
Gordon Dennis T.,
Kowalyshyn Doris E.,
Jensen Robert G.
Publication year - 1973
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533239
Subject(s) - lipolysis , lipase , chemistry , pancreatic lipase , hydrolysis , double bond , clinical chemistry , glycerol , glyceride , candida antarctica , hydrolase , lipidology , chromatography , organic chemistry , biochemistry , fatty acid , enzyme , adipose tissue
Abstract Fifteen triacylglycerols containing 12∶0, 14∶0, 16∶0, 18∶2 and one positional isomer of cis ‐18∶1 were hydrolyzed by pancreatic lipase (EC 3.1.1.3, glycerol ester hydrolase). The fatty acids in the products of lipolysis were identified and measured by gas liquid chromatography. The substrates containing the Δ2 through Δ7 isomers of 18∶1 were resistant to pancreatic lipolysis. These isomers accumulated in the di‐ and residual triacylglycerols and were diminished in the free fatty acids. The discrimination was greates against the Δ5 isomer.