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The acyl and Alk‐1‐enyl groups of the major phosphoglycerides from ox brain myelin and mouse brain microsomal, mitochondrial and myelin fractions
Author(s) -
Sun Grace Y.,
Horrocks Lloyd A.
Publication year - 1970
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533205
Subject(s) - microsome , chemistry , biochemistry , myelin , acylation , plasmalogen , chromatography , biology , phospholipid , enzyme , central nervous system , neuroscience , membrane , catalysis
The major phosphoglycerides from ox brain myelin and mouse brain microsomal, mitochondrial and myelin fractions were separated by preparative thin layer chromatography. Alk‐1‐enyl groups from the alk‐1‐enyl acyl glycerophosphorylethanolamines were reacted with 1,3‐propanediol to form the 1,3‐dioxolane derivatives. Acyl groups were converted to the methyl ester derivatives and the acyl groups from alk‐1‐enyl acyl glycerophosphorylethanolamines and diacyl glycerophosphorylethanolamines were also determined separately. The acyl and alk‐1‐enyl group compositions of the phosphoglycerides from microsomal and mitochondrial fractions were quite similar. The ethanolamine and serine phosphoglycerides contained large amounts of 18∶0, 18∶1, 20∶4 and 22∶6 acyl groups. The choline phosphoglycerides had small amounts of polyunsaturated acyl groups and large amounts of 16∶0, 18∶1 and 18∶0 acyl groups. The mitochondrial cardiolipins contained unusual amounts of several acyl groups including 18∶1, 52%; 18∶2, 6%; and 16.1, 4%. A large portion of the mouse brain 18∶2 is in that fraction. The myelin phosphoglycerides were deficient in saturated and 22∶6 groups and markedly enriched in 18∶1 and 20∶1 groups when compared with the corresponding microsomal or mitochondrial phosphoglycerides.