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Absorption and metabolism of 1‐ 14 C‐hydroxy octadecadienoate in the rat
Author(s) -
Reber R. J.,
Draper H. H.
Publication year - 1970
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533201
Subject(s) - chemistry , in vivo , metabolism , hydrolysis , triglyceride , biochemistry , adipose tissue , fatty acid , fatty acid metabolism , chromatography , biology , cholesterol , microbiology and biotechnology
The metabolism of 1‐ 14 C‐9(13)‐hydroxy octadecadienoic acid methyl ester (1‐ 14 C‐HAME) by the rat was investigated in vivo and in liver slices. A 1.5 mg dose of 1‐ 14 C‐HAME administered by stomach tube was efficiently hydrolyzed and absorbed from the intestinal tract. In comparison with 1‐ 14 C‐methyl linoleate (1‐ 14 C‐ML), 1‐ 14 C‐HMAE was more extensively oxidized to 14 CO 2 in vivo and in vitro. After 1‐ 14 C‐HAME administration as much as 50% of the radioactivity in the adipose tissue triglycerides was associated with 14 C‐hydroxy fatty acids. The remaining activity was present in randomly labeled normal fatty acids. No evidence was obtained for the incorporation of 14 C‐hydroxy acids into liver lipids; most of the radioactivity from 1‐ 14 C‐HAME in this organ was recovered in saturated and monoenoic fatty acids. About 10% of the radioactivity 24 hr after 1‐ 14 C‐HAME administration was associated with triglyceride trienoic acids, indicating that at least a portion of this acid was dehydrated in the liver. An unidentified polar acid was detected in the urine of the 1‐ 14 C‐HAME‐treated animals.