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Effect of oxidation on chemical spectral and immunochemical properties of egg yolk lipoprotein
Author(s) -
Hara I.,
Shimasaki H.,
Sato J.
Publication year - 1973
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533145
Subject(s) - ethylenediaminetetraacetic acid , chemistry , thiobarbituric acid , sonication , yolk , chromatography , nuclear chemistry , biochemistry , inorganic chemistry , chelation , lipid peroxidation , antioxidant , food science
Studies are reported on the changes in the chemical, spectral, and immunochemical properties of the low density lipoprotein fraction of hen's egg yolk during oxidation. Sonication for 15 min at 15–20 C in the presence or absence of iron gave an increase in thiobarbituric value and UV absorption but did not influence the fluorescence. Storage at 50 C in the absence of iron after sonication for 15 min at 15–20 C produced increases in absorptivity in the fluorescence and UV spectra, as well as thiobarbituric values. Similar changes were produced without sonication in the presence of iron in ca. 8 days storage at 50 C. Ethylenediaminetetraacetic acid completely inhibited the effect of copper, as well as iron, under these conditions. Immunoelectrophoresis pattern was effected by oxidation under conditions that gave an increase in absorptivity in the fluorescence spectra. The first change appeared to involve a minor fast moving component which decreased even when oxidation was inhibited by ethylenediaminetetraacetic acid. As the oxidation proceeded, the mobility of the major component in the immunoelectrophoresis analysis was altered. The major reaction affecting the immunochemical properties appeared to be between the product of lipid oxidation and the protein moiety.