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Specificity of Geotrichum candidum lipase with respect to double bond position in triglycerides containing cis ‐octadecenoic acids
Author(s) -
Jensen Robert G.,
Gordon Dennis T.,
Heimermann Wayne H.,
Holman Ralph T.
Publication year - 1972
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533124
Subject(s) - geotrichum , lipase , chemistry , double bond , hydrolysis , glyceride , enzyme , organic chemistry , stereochemistry , chromatography , fatty acid , food science
Fifteen approximately random, mixed, triacylglycerols, each of which contained 12∶0, 14∶0, 9, 12–18∶2, 16∶0 and only one positional isomer of cis 18∶1 (Δ2 through Δ16), were synthesized. These mixtures were used as substrates for the lipase from the microorganism Geotrichum candidum to define the specificity of the enzyme for unsaturated fatty acids. Comparatively small quantities of the 18∶1 isomers, other than 9–18∶1, were hydrolyzed. Relatively large amounts of 18∶2 were released from all substrates. There was no preference between 9–18∶1 and 18∶2. The positional isomers other than 9–18∶1 accumulated in the di‐ and mono‐acylglycerols.