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Lipolytic activities of intact walker 256 ascites tumor cells
Author(s) -
Cenedella Richard J.,
Imrich Bernard J.
Publication year - 1974
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533111
Subject(s) - lipolysis , fatty acid , biochemistry , clinical chemistry , ascites , phospholipid , in vitro , chemistry , adipose tissue , lactic acid , biology , medicine , bacteria , membrane , genetics
The present studies confirm with Walker carcinosarcoma 256 in ascites form that ascites tumor cells produce and release large quantities of free fatty acid when incubated in vitro under the appropriate conditions. Two distinct lipolytic acitivities were recognized; a cold sensitive acidic lipolytic activity and a heat sensitive neutral‐to‐weakly‐alkaline activity. Agents known to influence lipolysis in adipose tissue did not affect these activities. Glucose did inhibit the free fatty acid production at neutral pH, but this effect was apparently secondary to medium pH changes as a consequence of lactic acid production from glucose. These observations, together with finding a marked similarity between the composition of free fatty acid present in ascites fluid and those produced in vitro at both acid and neutral pH, are taken to support the concept that ascites tumor cells rapidly incorporate exogenous (medium) free fatty acid into a small and rapidly turning‐over fatty acid‐ester pool, probably a phospholipid pool.