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Lipid composition of rat superior cervical ganglion
Author(s) -
Organisciak D. T.,
Klingman J. D.
Publication year - 1974
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533106
Subject(s) - chromatography , chemistry , phosphatidylethanolamine , sphingomyelin , cerebroside , phosphatidylcholine , phospholipid , thin layer chromatography , silica gel , wax , oleic acid , silicic acid , stearic acid , biochemistry , cholesterol , organic chemistry , membrane
Excised rat superior cervical ganglion were incubated in Krebs‐Ringer solution, freeze‐dried, and lipids extracted with chloroform: methanol (2∶1) v/v. Chromatography of lipid extracts in three separate thin layer chromatography solvents was accomplished on a single thin layer chromatography plate coated with acid‐washed Silica Gel H. Individual lipids were eluted from the silica gel using a modified Swinny filter holder technique and transesterified with methanolic‐BF 3 . Fatty acid methyl esters were separated and quantitated by gas liquid chromatography, while phospholipids were determined with a Malachite green dye organic phosphorus assay. Quantitation of neutral lipids was accomplished with a micro Liebermann‐Burchard technique and sphingolipids estimated colorimetrically as free sphingosine. The composition of lipids from freezedried rat ganglia resembles the lipid composition of rat brain. Phosphatidylcholine and phosphatidylethanolamine represent 76% of the glycerolphosphatides. The sphingolipids were comprised primarily of sphingomyelin with moderate levels of cerebroside and sulfatide. Cholesterol was the predominant neutral lipid. The major phospholipid fatty acids included palmitic, stearic, and oleic acids.