Subfractionation of S f 4–10 5 , S f 4–20 and high density lipoproteins

Subfractionation of the total low density S f 4–10 5 , the low density S f 4–20 and high density plasma (or serum) lipoproteins has been accomplished using a cumulative flotation rate procedure. Fractionation employs nonlinear salt gradients and high performance swinging bucket rotors. Subfractionation of the total low density lipoproteins with minimal contamination allows and extremely accurate lipoprotein mass measurement of S f > 400, total very low density lipoproteins and low density lipoproteins (LDL) by elemented CHN analysis. Physical and chemical data on LDL and high density lipoprotein (HDL) subfractions are in general agreement with earlier data. Lower molecular weight data are obtained for HDL subfractions than reported earlier; however this may be the result of the different fractionation procedures used.