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Ratio of in vivo incorporation of 3 H arachidonic acid and 14 C linoleic acid into liver lipids from normal and diabetic rats
Author(s) -
Mercuri Osvaldo,
De Tomas Maria Elena
Publication year - 1971
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533039
Subject(s) - arachidonic acid , linoleic acid , chemistry , clinical chemistry , lipidology , diglyceride , medicine , biochemistry , endocrinology , fatty acid , in vivo , chromatography , enzyme , biology , microbiology and biotechnology
Normal and streptozotocin diabetic rats were injected via the portal vein with a labeled solution containing 3 H arachidonic acid and 14 C linoleic acid ( 3 H/ 14 C ratio, 0.5) during a 1 min period. Livers were quickly frozen, pulverized, and the lipids extracted and fractioned by thin layer chromatography. The incorporation of 3 H and 14 C into liver lipids was measured and the percentage distribution of radioactivity into the different lipid fractions was determined. The incorporation of 14 C linoleic acid and 3 H arachidonic acid into liver lipids is apparently reduced in rats with severe diabetes. The higher 3 H/ 14C ratio found in the 1,2 diglycerides from diabetic rats may be explained by the apparently smaller incorporation of 14 C linoleic acid or by an isotopic dilution attributable to the great availability of this acid in diabetic rats. On the other hand, the higher 3 H/ 14 C ratio observed in triglycerides and phospholipids from diabetic rats, due to a relatively large incorporation of 3 H arachidonic acid into this fraction, may be explained by the affinity of the enzymes involved in their synthesis for some 1,2‐diglyceride units. Insulin was unable to correct the changes observed in the diabetic rats.