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The effect of two isomeric octadecenoic acids on alkyl diacyl glycerides and neutral glycosphingolipids of Novikoff hepatoma cells
Author(s) -
Steele W.,
Jenkin H. M.
Publication year - 1972
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533024
Subject(s) - ceramide , glyceride , lipidology , alkyl , chemistry , clinical chemistry , sphingosine , biochemistry , chromatography , fatty acid , organic chemistry , apoptosis , receptor
The addition of 150 μg of cis ‐6‐octadecenoic acid (6–18∶1) per milliliter to the growth medium of Novikoff hepatoma cells reduced the rate of multiplication of these cells, whereas the same level of cis ‐9‐octadecenoic acid (9–18∶1) was without effect. The quantity of alkyl diacyl glycerols in cells grown in medium containing 100 μg/ml of either 6–18∶1 or 9–18∶1 was reduced to about 10% of that observed in cells grown in unsupplemented media. The presence of 6–18∶1 acid led to an increase in the concentration of the dihexosyl ceramide and a corresponding decrease in that of the monohexosyl ceramides of the cells; whereas the presence of 9–18∶1 resulted in an increase in the relative amount of tetraglycosyl ceramide and corresponding reductions in those of mono‐ and dihexosyl ceramide. Possible causes for these changes are discussed.