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The effect of temperature on the fatty acid and phospholipid composition of Cephalosporium falciforme and Cephalosporium kiliense
Author(s) -
Sawicki Edward H.,
Pisano Michael A.
Publication year - 1977
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532984
Subject(s) - silicic acid , phospholipid , chromatography , chemistry , sterol , fatty acid , thin layer chromatography , biochemistry , column chromatography , sephadex , polyunsaturated fatty acid , organic chemistry , cholesterol , membrane , enzyme
Abstract The effect of temperature on the lipid composition of Cephalosporium falciforme and Cephalosporium kiliense , causative agents of maduromycosis, was investigated. The fungi were grown at 28.5 C and 37 C in a chemically defined medium. The lipids were solvent extracted, purified on Sephadex, and separated into their component classes by silicic acid column chromatography. Five lipid classes were found: (a) sterol esters, (b) triacylglycerides, (c) free fatty acids, (d) sterols, and (e) phospholipids. Fatty acids were analyzed by gas liquid chromatography and phospholipids by thin layer chromatography. Temperature induced changes of varying degrees occurred in both the fatty acid phospholipid fractions of each organism.