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Uptake and metabolism of fatty acids by Soybean suspension cells
Author(s) -
Stumpf P. K.,
Weber N.
Publication year - 1977
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532983
Subject(s) - chemistry , phosphatidylcholine , oleic acid , biochemistry , stearic acid , palmitic acid , phosphatidylethanolamine , fatty acid , suspension culture , chromatography , metabolism , lipidology , phospholipid , organic chemistry , biology , cell culture , genetics , membrane
Soybean suspension cultures very rapidly take up C 16 and C 18 fatty acids by a nonspecific, nonenzymic binding of exogeneously added fatty acids to cell walls and by a subsequent transfer into the cell where they are rapidly incorporated into triacylglycerols, phosphatidylcholines, and phosphatidylethanolamines. 14 C‐Palmitic and 14 C‐stearic acids follow this sequence but are not desaturated, wherease 14 C‐oleic and 14 C‐linoleic acids are transferred more rapidly than the saturated fatty acids and are then further modified. All the data fit a sequence of events by which free oleic acid is first activated to a CoA thioester, and then desaturated to linoleyl‐CoA; both thioesters are then transferred to triacylglycerols, phosphatidylcholine, and phosphatidylethanolamine.