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Study of bound phospholipase activities of fungal mycelia using an organic solvent system
Author(s) -
Blain J. A.,
Patterson J. D. E.,
Shaw C. E.,
Akhtar M. Waheed
Publication year - 1976
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532901
Subject(s) - phospholipase , rhizopus arrhizus , chemistry , hydrolysis , chromatography , phosphatidylcholine , mycelium , diisopropyl ether , biochemistry , phospholipase a1 , enzyme , lysophospholipase , ether , lipase , organic chemistry , phospholipid , biology , botany , membrane
It has been possible to demonstrate and characterize high phospholipase activities in mycelia of Rhizopus arrhizus and Mucor javanicus by use of a system in which substrates were dissolved in diisopropyl ether. Such activities were associated with bound enzymes and would have been difficult to detect using aqueous assay systems. In both cases, phosphatidylcholine hydrolysis was by phospholipase A 1 (EC 3.1.1.32) activity followed by the action of lysophospholipase (EC 3.1.1.5). Phospholipase D (EC 3.1.4.4) activity was also detected. The methods used appear to be of general applicability for the detection and study of insoluble phospholipases.