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Effects of pentadecan‐2‐one on the growth of cells in culture
Author(s) -
Gilbertson John R.,
Fletcher Ronald D.,
Kawalek Joseph C.,
Demcisak Barbara
Publication year - 1976
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532854
Subject(s) - hela , trypan blue , incubation , cell growth , cell culture , growth inhibition , lysis , cell , biochemistry , alcohol , chemistry , clinical chemistry , ketone , microbiology and biotechnology , biology , organic chemistry , genetics
The effect of inclusion of trace amounts of pentadecan‐2‐one in the incubation medium on the growth of HeLa Cells was evaluated by measuring viable cell counts (cells excluding trypan blue) and incorporation of [ 14 C] leucine into acid precipitable protein. Evidence is presented to show that exposure of the cells to trace amounts of the methyl ketone, 36μg/ml, effectively inhibits cell growth. This inhibition is relieved by simultaneously incubating the cells with a long chain primary alcohol, hexadecan‐1‐ol, but not with the secondary alcohol, pentadecan‐2‐ol. The observation that the ethyl ketone, hexadecan‐3‐one, also inhibits cell growth but at higher concentrations than that observed with pentadecan‐2‐one and that pentadecan‐2‐ol at similar concentrations has no effect on cell growth indicates that, for optimal effect, the keto function must be at the 2‐position. Inhibition of cell growth by pentadecan‐2‐one is not unique to HeLa cells, as suggested by the inhibitory effects of this lipid type on the growth of other malignant cell lines of human origin.