Premium
Isolation of human serum low‐density lipoproteins with the aid of an immune‐specific adsorber
Author(s) -
Kostner Gerhard,
Holasek Anton
Publication year - 1970
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532735
Subject(s) - chemistry , chromatography , very low density lipoprotein , ultracentrifuge , lipoprotein , radial immunodiffusion , low density lipoprotein , biochemistry , electrophoresis , antibody , cholesterol , biology , immunology
Human serum lipoproteins containing B‐protein have been isolated using an immunoadsorber. Bromoacetyl cellulose was combined with pure antibodies to low density lipoprotein (LDL) and an immunoadsorber of high capacity was obtained. With 1 g of this immunoadsorber all LDL and very low density lipoprotein (VLDL) from 30 ml pooled human serum were adsorbed and then eluted with glycine‐HCl buffer pH 3.2 at 0 C. The isolated lipoproteins were investigated by electrophoresis, immunodiffusion and ultracentrifugation, and found to be identical to LDL+VLDL isolated by ultracentrifugation.