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Methodology for separation of gangliosides from potential water‐soluble precursors
Author(s) -
Carter T. P.,
Kanfer J.
Publication year - 1973
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532709
Subject(s) - chromatography , chemistry , trichloroacetic acid , bovine serum albumin , silicic acid , ganglioside , nucleotide , biochemistry , organic chemistry , gene
The following methods were examined for their ability to remove labeled nucleotide sugars and other water‐soluble precursors from ganglioside preparations: enzymatic digestion of nucleotide sugars followed by dialysis; silicic acid and Sephadx G‐25 column chromatography; ascending paper chromatography; high voltage paper electrophoresis; thin layer chromatography; co‐precipitation of gangliosides and bovine serum albumin with trichloroacetic acid. A procedure was developed which allows these complex glycosphingolipids to remain in the chloroform‐rich lower phase during conventional Folch partitioning.