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Lipid products formed during desaturation of [1‐ 14 C] stearyl CoA by hen liver microsomes
Author(s) -
Holloway Caroline T.,
Holloway P. W.
Publication year - 1974
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532692
Subject(s) - oleic acid , lipidology , clinical chemistry , microsome , chemistry , chromatography , oleyl alcohol , biochemistry , lipid metabolism , organic chemistry , enzyme
Abstract A number of lipid products are formed during the desaturation of stearyl CoA by hen liver microsomes. This article presents an analysis of the products formed when [1‐ 14 C] stearyl CoA is incubated with hen liver microsomes for various time periods. [1‐ 14 C] Oleyl CoA was the first radioactive unsaturated product formed. Synthesis of phospholipids containing [1‐ 14 C] oleic acid occurs only after the desaturase activity has begun to decline. The specific radioactivity of [1‐ 14 C] oleyl CoA was similar to the specific radioactivity of [1‐ 14 C] stearyl CoA at all time periods tested. The specific radioactivities of [1‐ 14 C] oleic acid and phospholipids containing [1‐ 14 C] oleic acid were much lower than that of the [1‐ 14 C] stearyl CoA.