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Characteristics of the lipase from the mold, Geotrichum candidum : A review
Author(s) -
Jensen Robert G.
Publication year - 1974
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532686
Subject(s) - geotrichum , lipase , degree of unsaturation , chemistry , hydrolysis , enzyme , triglyceride , chromatography , organic chemistry , biochemistry , food science , cholesterol
The mold Geotrichum candidum produces an extracellular lipase, readily concentrated by removal of the culture medium in which the microorganism is grown. The lipase is characterized by a unique, but not absolute, specificity for fatty acids containing cis ‐9 or cis,cis ‐9, 12 unsaturation, hydrolyzing both regardless of position within the triglyceride molecule. The enzyme also hydrolyzes cis ‐9‐16∶1, cis,trans ‐9,12‐18∶2, trans,cis ‐9,12‐18∶2, palmitoyl oleate and cholesteryl oleate. Digested at comparatively slow rates are: trans,trans ‐9,12‐18∶2, double bond positional isomers of 18∶1 (other than cis ‐9), stearolic acid, oleoylpalmitate, dilinoleoyl phosphatidyl choline, and saturated acids. The enzyme has an optimum pH of 8.2, and the lyophilized powder is extremely stable, retaining activity for at least eight years when stored at‐20 C. A purification of 81‐fold has been achieved.