Premium
Low temperature direct methylation of lipids in biological materials
Author(s) -
Dugan L. R.,
McGinnis Gertrude W.,
Vadehra D. V.
Publication year - 1966
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532671
Subject(s) - methylation , chemistry , methanol , chromatography , blood lipids , biochemistry , sulfuric acid , fatty acid , organic chemistry , ether , yeast , cholesterol , gene
The procedure for low temperature methylation of fatty acids in lipids by sulfuric acid‐methanol has been adapted to direct methylation of fatty acids of lipids in biological materials without prior extraction of the lipids. Successful application requires a solution or a suspension of fine particles of the lipid bearing material in ether. Concentrated sulfuric acid is added to the solution or suspension at low temperatures followed by addition of absolute methanol. The acid is neutralized by methanolic KOH and the esters extracted. Application of the method to prepare methyl esters of lipids in cream, blood serum, swine liver and kidney tissue, and cells of yeast on Staphylococcus aureus show that fatty acid composition based on this method compares with that determined by methylation of extracted lipids.