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Is there an entero‐hepatic circulation of the bile phospholipids?
Author(s) -
Boucrot Philippe
Publication year - 1972
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532645
Subject(s) - oleic acid , phospholipid , chemistry , bile acid , lipidology , hydrolysis , biochemistry , clinical chemistry , fatty acid , chromatography , duodenum , small intestine , tritium , medicine , physics , membrane , nuclear physics
Bile previously labeled with tritiated oleic acid (the main radioactivity was on bile phospholipids) was mixed with pure isolated phospholipids previously labeled with 14 C oleic acid; this mixture was perfused during 6 or 23 hr into the duodenum of test rats bearing a bile fistula. At the time of decapitation, in the small intestine a large hydrolysis of the 14 C phospholipids was found. In contrast no bile phospholipid hydrolysis was observed. In the collected bile samples of the test rats, no 14 C could be detected (this means a very large decrease of the 14 C fatty acids specific activities by the body fatty acids), and the tritiated fatty acids specific activities were only 2.5–12 times lower than in the perfused bile. These results can be explained, assuming that the bile phospholipids enter in an entero‐hepatic circulation and are preserved from the dilution in a large pool of lipids.