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Serum lipoproteins: A paper electrophoresis method without albumin in the buffer
Author(s) -
Moinuddin Mohammed,
Taylor Linnard
Publication year - 1969
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532627
Subject(s) - chromatography , electrophoresis , chemistry , albumin , staining , chylomicron , lipoprotein , sudan black b , buffer (optical fiber) , bovine serum albumin , gel electrophoresis , very low density lipoprotein , biochemistry , cholesterol , biology , telecommunications , computer science , genetics
A paper electrophoresis method is described in which four serum lipoprotein components are separated without the use of albumin in the buffer. Tris‐EDTA‐boric acid buffer, Oil Red O staining solution prepared in 52.8% instead of the usual 60% alcohol, and solvent‐extracted paper strips are the distinguishing features of this procedure. The system was effective not only in separating chylomicron, β‐ and α‐lipoproteins into well‐defined bands, but also in separating very low density or pre‐β‐lipoprotein distinctly in some samples and partially in others. The advantages of this procedure are low background staining and, in comparison to the procedure using albumin in the buffer, a sharper alpha band. This sharpened α‐band gives a sharper peak in densitometric scanning. Electrophoresis could be performed for 16 hr or 4 hr. The 4‐hr electrophoresis run produced electrophoregrams with even denser and sharper bands than the 16‐hr run.