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Inhibition of the plasma lecithin‐cholesterol acyltransferase reaction by hydrogen peroxide and peroxidized lecithin
Author(s) -
Mickel Hubert S.,
Foulds Emmett L.,
Clark Dale A.
Publication year - 1972
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532599
Subject(s) - chemistry , hydrogen peroxide , lecithin , acyltransferase , sterol o acyltransferase , biochemistry , enzyme , cholesterol , clinical chemistry , lecithin—cholesterol acyltransferase , peroxide , chromatography , organic chemistry , lipoprotein
Inhibition of the plasma lecithincholesterol acyltransferase reaction by hydrogen peroxide and peroxidized lipids was studied by two methods: a radiochemical tracer technique and a direct colorimetric assay. Protection of sulfhydryl groups with p ‐chloromercuribenzoate, prior to exposure to peroxide, resulted in restoration of acyltransferase activity on removal of the inhibitors with mercaptoethanol. On the basis of these observations, it is proposed that lipid peroxides inhibit the plasma lecithin‐cholesterol acyltransferase reaction by alteration of sulfhydryl groups necessary for enzymatic activity.