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In vitro activity of the fatty acyl desaturases of human cancerous and noncancerous tissues
Author(s) -
Nakazawa Ichiro,
Mead James F.,
Yonemoto Robert H.
Publication year - 1976
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532589
Subject(s) - in vitro , arachidonic acid , chemistry , clinical chemistry , urinary system , biochemistry , microsome , cancer , fatty acid , substrate (aquarium) , oleic acid , linoleic acid , biology , medicine , enzyme , endocrinology , ecology
The microsomal desaturase activity of human cancerous and noncancerous tissues was measured in vitro using 1‐ 14 C,‐11,14‐eicosadienoic and 1‐ 14 C‐oleic acids as substrates. Tissues used were a case of ovarian cancer, a urinary bladder cancer, a rectal cancer, and a nonspecific colonic ulcer with appropriately normal tissues. When 11,14–20∶2 was used as substrate, radioactive tetraene and triene were produced. The tetraene was identified by radio gas chromatography as arachidonic acid (5,8,11,14–20∶4), and the triene had a retention time of 5,11,14–20∶3. Thus, the possibility arises that a Δ8 desaturase was involved. In the Δ6 desaturase, with the urinary bladder