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A micromethod for the stereospecific determination of triglyceride structure
Author(s) -
Lands William E. M.,
Pieringer Ronald A.,
Slakey Sister P. M.,
Zschocke Albrecht
Publication year - 1966
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532550
Subject(s) - diglyceride , triglyceride , stereospecificity , triglyceride lipase , clinical chemistry , chemistry , lipidology , biochemistry , pancreatic lipase , lipase , glycerol kinase , fatty acid , enzyme , cholesterol , catalysis
Triglyceride lipase and diglyceride kinase can be used in a sensitive stereospecific analysis of the separate fatty acid compositions at the 1, 2 and 3 positions of a triglyceride. Diglyceride kinase from Escherichia coli selectively catalyzes the phosphorylation of 1,2‐diglycerides but not the 2,3‐diglycerides. The composition of the 3‐position in rat liver triglycerides is clearly different from that at the 1‐position.