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Free and protein‐bound gangliosides in triton X‐100 extracts from rat brain
Author(s) -
Brunngraber Eric G.,
Ziboh Vincent A.
Publication year - 1974
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532515
Subject(s) - ganglioside , chemistry , microsome , biochemistry , chromatography , clinical chemistry , lipidology , yield (engineering) , mitochondrion , enzyme , materials science , metallurgy
Gangliosides that are extracted from brain tissue samples with Triton X‐100 exist in a free form and as a ganglioside‐protein complex. The two forms can be separated by diethylaminoethyl‐cellulose chromatography. Whole brain tissue yields only ganglioside‐protein complexes. Crude mitochondrial and microsomal fractions and synaptosome‐enriched fractions yield both free and protein‐bound gangliosides. The myelin and mitochondria‐enriched fractions yield only free gangliosides. The isolation of ganglioside‐protein complexes and free gangliosides appears to be dependent upon the nature of the starting material.