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Electron spin resonance study of serum lipoproteins of salmon ( Oncorhynchus kisutch ): Structural alterations produced in high density lipoproteins by mercury and cadmium
Author(s) -
Reichert William L.,
Malins Donald C.
Publication year - 1975
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532489
Subject(s) - chemistry , electron paramagnetic resonance , high density lipoprotein , lipoprotein , clinical chemistry , low density lipoprotein , spin density , biochemistry , cholesterol , nuclear magnetic resonance , physics , condensed matter physics
High denisty lipoproteins from the serum of salmon ( Oncorhynchus kisutch ), containing a maleimide spin label (4‐male‐imido‐2,2,6,6‐tetramethylpiperidinooxyl), were exposed to HgCl 2 , CdCl 2 , and CH 3 HgCl under physiological conditions. Electron spin resonance spectrometry revealed that HgCl 2 and CdCl 2 produce significant alterations at sites representing weakly and strongly immobilized label. The observed changes appear to be taking place primarily in the surface architecture. No alterations were found in the normal spectra of high density lipoproteins exposed to CH 3 HgCl under similar conditions. Each metal species binds strongly with the apo‐high density lipoproteins; however, marked differences exist in the effects produced upon high density lipoprotein structure by the inorganic ions and CH 3 HgCl.