Premium
In vivo transfer of 14 C‐4‐cholesterol from mouse host to encapsulated Trichinella spiralis larvae
Author(s) -
Digenis George A.,
Konyalian Arpine,
Thorson Ralph
Publication year - 1970
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532481
Subject(s) - trichinella spiralis , cholesterol , thin layer chromatography , larva , trichinella , in vivo , parasite hosting , biology , lipidology , trichinosis , host (biology) , clinical chemistry , chromatography , biochemistry , microbiology and biotechnology , chemistry , helminths , botany , immunology , ecology , world wide web , computer science
Mice infected for four months with the parasite Trichinella spiralis were fed 4‐ 14 C‐cholesterol. The encapsulated larvae were then collected from the mice, and the lipids of the larvae were extracted and fractionated by thin layer chromatography (TLC). One of the TLC bands known to contain cholesterol was subfractionated; the resulting bands were assayed for radioactivity. It was found that the pure cholesterol fraction contained radioactive cholesterol, presumably from the host.