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Erucic acid metabolism by rat heart preparations
Author(s) -
Cheng Chakki,
Pande Shri V.
Publication year - 1975
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532455
Subject(s) - erucic acid , carnitine , rapeseed , beta oxidation , palmitic acid , clinical chemistry , lipidology , chemistry , acyl coa , biochemistry , metabolism , mitochondrion , medicine , endocrinology , fatty acid , food science , biology , enzyme
Rat heart preparations metabolized erucic acid at much slower rates than palmitic acid. This applied for activation reaction, for the conversion of acyl‐CoA to acylcarnitine, and for the utilization of acyl group for oxidation. As compared to palmityl‐CoA, erucyl‐CoA exhibited a lower affinity for carnitine palmityltransferase (EC 2.3.1 23), the respective apparent Michaelis constants were 43 and 83 μM. Presence of erucyl‐CoA or erucylcarnitine slowed the mitochondrial oxidation of palmityl groups apparently because of the slower oxidation of erucyl groups. However, presence of erucate did not inhibit the activation of palmitate. Heart mitochondria obtained from rats fed rapeseed oil (50 cal %) or corn oil diet for 3 days showed similar abilities for the coupled oxidation of various substrates and similar carnitine palmityltransferase activities. Thus a suggestion of gross mitochondrial malfunction following rapeseed oil consumption was not confirmed.