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Phospholipase C catalyzed formation of sphingomyelin‐ 14 C from lecithin and N‐( 14 C)‐oleoyl‐sphingosine
Author(s) -
Kanfer Julian N.,
Spievlogel Christine H.
Publication year - 1975
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532442
Subject(s) - choline , chemistry , sphingomyelin , lecithin , cytidine , phospholipase d , biochemistry , phospholipase , phosphatidylcholine , ceramide , enzyme , membrane , phospholipid , apoptosis
Commercial preparations of Clostridium perfringens were incubated with phosphatidyl choline and N‐1‐( 14 C) oleoylsphingosine. A radioactive product was formed which cochromatogramed with sphingomyelin standard in three different solvent systems. Several other phospholipases and phosphatases were unable to catalyze this reaction. Neither choline, phosphoryl choline, cytidine diphosphate choline nor p‐nitrophenyl phosphoryl choline were active donors. Sphingomyelin was only slightly active as a phosphoryl choline donor.