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Incorporation and metabolism of fatty acids by cultured dissociated cells from rat cerebrum
Author(s) -
Yavin Ephraim,
Menkes John H.
Publication year - 1974
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532201
Subject(s) - linolenate , biochemistry , stearic acid , phospholipid , fatty acid , metabolism , oleic acid , lipidology , ethanolamine , chemistry , incubation , clinical chemistry , stearate , linolenic acid , biology , organic chemistry , linoleic acid , membrane
Dissociated brain cells in culture incorporate a variety of saturated and unsaturated fatty acids into their cellular lipids. Of the various fatty acids studied, uptake of radioactivity was greatest for stearic acid and decreased progressively with decreasing chain length. Incorporation of radioactivity from linoliec and linolenic acids was more extensive than from oleic acid. Cellular phospholipids and triacylglycerols were labeled preferentially from all fatty acid precursors, with the relative amount of label in phospholipids being greatest when cells were incubated with linolenic acid. Fatty acids underwent desaturation and chain elongation. Changes in the labeling pattern of phospholipid fatty acids in the course of incubation demonstrated precursor‐product relationships for laurate (12∶0), myristate (14∶0), palmitate (16∶0), and stearate (18∶0) and for linolenate (18∶3), eicosapentaenoate (20∶5), docosapentaenoate (22∶5), and docosahexaenoate (22∶6). The appearance of label in 22∶5 and 22∶6 paralleled the entrance of label into the ethanolamine phosphoglyceride fraction. Conversion of linoleate (18∶2 ω6) to arachidonate (20∶4 ω6) could be demonstrated but did not proceed via 18∶3 ω6.