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Glycosphingolipids of white cells lymphoid tissue and bone marrow
Author(s) -
Levis G. M.,
KesseElias M.
Publication year - 1974
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532170
Subject(s) - glycosphingolipid , bone marrow , glycolipid , lymphatic system , ceramide , chemistry , biology , population , phospholipid , granulocyte , lymphocyte , biochemistry , immunology , membrane , medicine , apoptosis , environmental health
Abstract Glycosphingolipids of lymphoid tissues, bone marrow cells, mixed blood leucocytes, separated lymphocytes, and granulocytes from large white pigs were analyzed by thin layer and gas liquid chromatographies. The composition of the glycosphingolipids of thymus and blood leucocytes (mixed population) was similar, and trihexosyl ceramide (galactosyl‐[1–4]‐galactosyl‐[1–4]‐glucosyl‐[1–1]‐ceramide) was the major glycosphingolipid component of both tissues. The fatty acid fractions of all glycolipids from the two tissues were analyzed, and gross differences are discussed. Blood lymphocytes had a higher content of glycosphingolipids/mole phospholipid or mg protein than thymus lymphocytes obtained by a gentle washing of sliced tissue. Similar and more pronounced differences were obtained when the glycosphingolipid content of bone marrow cells (>50% polymorphonuclear neutrophils) was compared with that of blood polymorphonuclear neutrophils. In general, most of the blood leucocytes were richer in glycosphingolipids than most of the cells of the lymphoid tissues and bone marrow. These results indicate a marked difference in lipid composition between blood polymorphonuclear neutrophils and bone marrow cells. It is possible that certain of the biophysical properties which characterize blood polymorphonuclear neutrophils and which derive from changes in the cell periphery of immature granulocytes are connected with these differences in membrane lipid composition.