Premium
Differential trypsin effect upon (1‐ 14 C) acetate incorporation into choline and ethanolamine glycerophosphatides of rat brain and liver
Author(s) -
Cohen Steven R.,
Bernsohn Joseph
Publication year - 1974
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532128
Subject(s) - ethanolamine , choline , chemistry , biochemistry , trypsin , chromatography , albumin , bovine serum albumin , bicarbonate , enzyme , organic chemistry
Preincubation of rat brain and liver slices in a medium (5% glucose, 5% fructose, 1% albumin, 1% trypsin, 10 mM phosphate buffer pH 6.0) used to pretreat brain tissue for the separation of cell types was found to uncouple the incorporation of (1‐ 14 C) acetate into ethanolamine phosphoglycerides from that of the choline phosphoglycerides. Incorporation into ethanolamine phosphoglycerides was stimulated in both brain (330%) and liver (780%) slices, while the incorporation of (1‐ 14 C) acetate into choline phosphoglycerides was reduced for both brain (71%) and liver (63%) slices, compared to control values from nonpreincubated material. With (1‐ 14 C) linolenic acid as a precursor, no significant differences were found in incorporation into ethanolamine phosphoglycerides and choline phosphoglycerides.