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Spectrophotometric determination of molar amounts of glycosphingolipids and ceramide by hydrolysis and reaction with trinitrobenzenesulfonic acid
Author(s) -
Yamamoto Akira,
Rouser George
Publication year - 1970
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532112
Subject(s) - chemistry , moiety , hydrolysis , ceramide , chromatography , cerebroside , organic chemistry , acid hydrolysis , molar ratio , biochemistry , catalysis , apoptosis
Three spectrophotometric procedures for determination of chromatographically separated glycosphingolipids (cerebroside, sulfatide, ganglioside) are described and compared. Hydrolysis to release long chain base followed by reaction of the amino group with trinitrobenezene sulfonic acid (TNBS) and measurement at 340 mμ was shown to give values in good agreement with those obtained by reaction of the carbohydrate moiety with α‐naphthol or anthrone and measurement at 550 and 625 mμ respectively. The TNBS method is also applicable to ceramide.