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Prostaglandins of rat testis
Author(s) -
Carpenter Mary P.
Publication year - 1974
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532057
Subject(s) - prostaglandin , chemistry , chromatography , fatty acid , thin layer chromatography , prostaglandin e2 , prostaglandin e , biochemistry , biology , endocrinology
The purpose of the study was to determine whether prostaglandins were present in mammalian testis, a tissue that has a large concentration of polyenoic fatty acids that are potential precursors of prostaglandins. Acid‐soluble lipids of rat testis were extracted, purified, and fractionated by thin layer and column chromatographies. 3 H‐Prostaglandins were added as internal reference standards to monitor recoveries and facilitate identification. Initial identification of prostaglandin species was done by chromatography. Further identification was done by elution of the prostaglandin zones followed by rechromatographies (both thin layer and column), measurements of UV absorption spectra, and by gas liquid chromatography. The results of these analyses indicate that prostaglandin E 1 , 11α,15(S)‐dihydroxy‐9‐oxo‐β‐ trans ‐prostenoic acid; prostaglandin E 2 , 11α‐15(S)‐dihydroxy‐9‐oxo‐5‐ cis ‐13‐ trans ‐prostadienoic acid; and prostaglandin F 1 , 9α,11α,15(S)‐trihydroxy‐13‐ trans ‐prostenoic acid occur in rat testicular tissue and that prostaglandin F 2α , 9α, 11α, 15(S)‐trihydroxy‐5‐ cis ‐13‐ trans ‐prostadienoic acid and prostaglandin E 2 , 11α,15(S)‐dihydroxy‐9‐oxo‐5‐ cis ‐13‐ trans ‐prostadienoic acid may be the primary species of this tissue. Prostaglandin B 1 , 15(S)‐hydroxy‐9‐oxo‐8(12),13‐ trans ‐prostadienoic acid and prostaglandin B 2 , 15(S)‐hydroxy‐9‐oxo‐5‐ cis ,8(12),13‐ trans ‐prostatrienoic acid also were detected, and some evidence was obtained for the presence of prostaglandin metabolites.