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Separation of cholesterol‐desmosterol acetates by thin‐layer and column chromatography on silica gel G‐silver nitrate
Author(s) -
Galli Giovanni,
Paoletti Enrica Grossi
Publication year - 1967
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02532004
Subject(s) - desmosterol , silver nitrate , chromatography , chemistry , silica gel , thin layer chromatography , cholesterol , sterol , organic chemistry , biochemistry
A chromatographic method, using columns packed with Celite‐Silica Gel G impregnated with silver nitrate, has been developed for the separation of cholesterol from desmosterol (24‐dehydrocholesterol). The system employed allows a complete separation of the two sterols as acetates, as determined by gas‐liquid chromatography and thin‐layer chromatography. The recovery of pure cholesterol and desmosterol is quantitative.