Premium
Mechanism of lipoxidase reaction. II. Origin of the oxygen incorporated into linoleate hydroperoxide
Author(s) -
Dolev Ami,
Rohwedder W. K.,
Mounts T. L.,
Dutton H. J.
Publication year - 1967
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531997
Subject(s) - chemistry , oxygen , molecule , aqueous two phase system , organic chemistry , conjugated system , aqueous solution , phase (matter) , polymer
Two different series of experiments were performed to establish the origin of the oxygen molecule incorporated into hydroperoxide during the incubation of lipoxidase with linoleic acid. These showed, as previously assumed but never demonstrated, that the oxygen introduced into the hydroperoxide molecules comes from the gaseous phase and not from the aqueous phase. Furthermore, soybean lipoxidase does not catalyze the exchange between gaseous oxygen and water oxygen. Possibly, lipoxidase may be involved in the biosynthesis of hydroxy trans,cis conjugated octadecadienoates present in various seeds.