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Bis‐(monoacylglyceryl)‐phosphate of rat and human liver: Fatty acid composition and NMR spectroscopy
Author(s) -
Wherrett J. R.,
Huterer S.
Publication year - 1973
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531989
Subject(s) - lipidology , chemistry , clinical chemistry , phosphate , nuclear magnetic resonance spectroscopy , glycerol , fatty acid , mass spectrometry , chromatography , biochemistry , spectroscopy , organic chemistry , physics , quantum mechanics
Bis‐(monoacylglyceryl)‐phosphate was isolated from liver lysosomes that had been prepared from rats pretreated with Triton WR‐1339. Analysis of the fatty acids in this lipid by gas chromatography and mass spectrometry revealed that they were composed of 69% docosahexenoic acid. NMR spectroscopy of bis‐(monoacylglyceryl)‐phosphate isolated from liver of a patient who had a lipid storage disease revealed that the peak corresponding to the chemical shift of protons of esterified secondary carbons of glycerol was absent. This suggests that fatty acids are esterified to the primary position of both glycerols.