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Application of silica gel‐sintered plate to thin layer lipid chromatographic analysis
Author(s) -
Itoh T.,
Tanaka M.,
Kaneko H.
Publication year - 1973
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531901
Subject(s) - lipidology , chromatography , silica gel , thin layer chromatography , layer (electronics) , clinical chemistry , thin layer , materials science , chemistry , composite material , biochemistry
The merits and disadvantages of using the silica gel‐sintered plate for lipid chromatographic analysis were investigated in detail. The commercially available sintered plate could be used repeatedly, employing the reconditioning procedure which involved chromic‐sulfuric acid treatment and subsequent activation. The reconditioned sintered plate has now been used successfully 20 times, for lipid analysis without any deterioration of the excellent resolution power for complex lipid mixtures for all the solvent systems. Since the sintered plate is sturdy, the chromatogplate could be immersed directly in the liquid reaction mixture, so that spots on the chromatogram could be seen and impregnated plates prepared simply. The sintered plate was found to be much more economical than the silica gel‐coated plate.