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Fractionation of triglyceride mixtures by preparative gas chromatography
Author(s) -
Kuksis A.,
Ludwig J.
Publication year - 1966
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531873
Subject(s) - chromatography , chemistry , gas chromatography , flame ionization detector , fractionation , analytical chemistry (journal) , triglyceride , hydrogen , splitter , organic chemistry , physics , biochemistry , cholesterol , optics
A semiautomatic system is described for gas‐chromatographic separation and recovery of triglycerides of uniform molecular weight in milligram quantities. It employs an Aerograph Autoprep 700 (Wilkens Instrument and Research Inc.) equipped with a stream splitter and a hydrogen flame ionization detector. The column is an aluminum or stainless steel tube (1/4 in. O.D.×2 ft) and contains silanized Chromosorb W (60–80 mesh) coated with 5% (w/w) JXR or SE‐30. Five to ten milligrams of mixed triglyceride are injected at a time and the temperature is programmed exponentially from 150 to 350C. With split ratios of 1∶5 to 1∶10 collections of 20 to 50 mg of each peak can be made with some 10 to 20 injections.