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Digestion of butyrate glycerides by pancreatic lipase
Author(s) -
Sampugna J.,
Quinn J. G.,
Pitas R. E.,
Carpenter D. L.,
Jensen R. G.
Publication year - 1967
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531853
Subject(s) - glyceride , chemistry , lipase , digestion (alchemy) , butyric acid , triglyceride , triolein , butyrate , fatty acid , biochemistry , diglyceride , hydrolysis , palmitic acid , triacylglycerol lipase , chromatography , enzyme , cholesterol , fermentation
The racemic triglycerides, glyceryl‐1‐palmitate‐2,3‐dibutyrate (PBB), glyceryl‐1‐butyrate‐2,3‐dipalmitate (PPB), glyceryl‐2‐butyrate‐1,3‐dipalmitate (PBP), and the diglyceride, racemic glyceryl‐1‐palmitate‐3‐butyrate (P‐B) were synthesized and digested with pancreatic lipase. Each triglyceride was mixed with equimolar amounts of triolein (OOO) prior to incubation. The following order of digestion rates was observed: PBB>PPB>PBP>P‐B. There was no evidence for short‐chain fatty acid specificity; however the triglycerides containing butyric acid were hydrolyzed more rapidly than OOO. Based upon the fatty acid composition of partial glycerides, digestion of butyrate glycerides was not a simple phenomenon. For example, in the digestion of PBB, butyric acid accumulated faster than palmitic acid in the diglycerides, and monobutyrin was found to accumulate when the diglyceride, P‐B, was digested. As evidenced by the fatty acid composition of the monoglycerides, positional specificity of pancreatic lipase was always maintained.