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Regulatory function of mitochondria in lipogenesis
Author(s) -
Harris Robert A.,
Leland Michael C.,
Mahoney Joan Munroe,
Mapes James P.
Publication year - 1973
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531837
Subject(s) - mitochondrion , lipogenesis , cytoplasm , lipidology , clinical chemistry , biochemistry , fatty acid synthesis , fatty acid , biology , cell , lipid metabolism , chemistry , microbiology and biotechnology
A cell‐free system, consisting of particle‐free rat liver cytoplasm plus purified mitochondria, was used to study the synthesis of fatty acid from 14 C‐U‐alanine. Incorporation of label into lipid was dependent upon the presence of both cytoplasm and mitochondria. However, the metabolic state of the animal, prior to sacrifice, determined the capacity of various mitochondrial preparations to reconstitute the system. Mitochondria prepared from the livers of starved, fat‐fed, or alloxan‐diabetic rats failed to support fatty acid synthesis to the same extent as mitochondria prepared from control animals. Likewise, less 14 CO 2 was produced in the system reconstituted with liver mitochondria prepared from starved rats. Possible mechanisms are discussed.