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In vivo incorporation of 3 H arachidonic acid and 14 C linoleic acid into liver lipids from essential fatty acid‐deficient rats
Author(s) -
Mercuri Osvaldo,
Elena De Tomás Maria
Publication year - 1973
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531814
Subject(s) - arachidonic acid , lipidology , linoleic acid , clinical chemistry , essential fatty acid , phospholipid , fatty acid , biochemistry , chemistry , in vivo , chromatography , biology , enzyme , membrane , microbiology and biotechnology
Essential fatty acid (EFA)‐deficient rats were injected intraportally with a labeled solution containing 3 H arachidonic acid and 14 C‐linoleic acid during a 1 min period. Livers were quickly frozen, pulverized, and the lipids extracted and fractioned by thin layer chromatography. The incorporation of 3 H and 14 C into liver lipids was measured, and the per cent distribution of radioactivity into the different lipid fractions determined and compared with those previously obtained from normal rats. In contrast with normal rats, ca. 70% of the 3 H arachidonic acid and 14 C‐linoleic acid incorporated into total lipids from EFA‐deficient rats was recovered in the phospholipid fraction. From the results of this experiment, it is suggested that a more active deacylation‐reacylation cycle in EFA‐deficiency could be responsible for this increase.