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Comparison of lipoprotein analysis by agarose gel and paper electrophoresis with analytical ultracentrifugation
Author(s) -
Noble R. P.,
Hatch F. T.,
Marzimas J. A.,
Lindgren F. T.,
Jensen L. C.,
Adamson G. L.
Publication year - 1969
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531795
Subject(s) - agarose , chromatography , electrophoresis , ultracentrifuge , chemistry , agarose gel electrophoresis , lipoprotein , gel electrophoresis , biochemistry , cholesterol , dna
A comparison has been made of human serum lipoprotein analysis by agarose gel and paper electrophoresis with a standard method of analytical ultracentrifugation. Samples were obtained from 28 patients with various disorders of lipoprotein metabolism. Correspondence was shown between the following electrophoretic and ultracentrifugal fractions: β and S f 0–20; pre‐β and S f 20–400; α 1 and total HDL. The deviations observed with the electrophoretic methods, though sizable, were smaller than the usual clinically significant abnormalities. Semiquantitative application is there fore justified. Agarose gel electrophoresis is slightly more difficult than paper electrophoresis, but gives improved resolution of pre‐β‐ and β‐lipoproteins and better densitometric scans. Evidence was also presented that the agarose method, when used in conjuction which ultracentrifugation, may be a valuable research technique for the study of lipoprotein properties.