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Effects of hydrogen peroxide on lipoproteins and associated lipids
Author(s) -
Clark Dale A.,
Foulds Emmett L.,
Wilson Fredrick H.
Publication year - 1969
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531786
Subject(s) - chemistry , ultracentrifuge , chromatography , cholesterol , lipoprotein , phospholipid , cholesteryl ester , elution , biochemistry , in vivo , hydrogen peroxide , phosphatidylcholine , biology , microbiology and biotechnology , membrane
Lipoproteins isolated from human or chimpanzee serum were treated with H 2 O 2 and allowed to stand varying lengths of time before quantitative analysis in the ultracentrifuge. Marked instability of ultracentrifugal boundaries (convection) occurred during the first 24 hr, but diminished thereafter. Simultaneously, the quantity of lipoprotein decreased. The instability of boundaries in H 2 O 2 ‐treated samples was presumed to reflect loss of lipid‐protein affinity and breakdown of lipoproteins under the force of ultracentrifugation. Analysis of extracted lipids showed that H 2 O 2 caused little loss of phospholipid, significant loss of triglyceride, and apparent loss of cholesteryl ester. The latter loss, however, was accompanied by appearance of esterified cholesterol in the free cholesterol eluent. Apparently H 2 O 2 converted some cholesteryl esters to a more polar form which was eluted later from the column, with the free cholesterol fraction. Gas chromatographic analysis of the fractions eluted from the column showed that selective degradation of polyunsaturated fatty acids was most marked with cholesteryl esters, somewhat less with triglycerides, and negligible with phospholipids. It was postulated that the loss of lipid‐protein affinity caused by H 2 O 2 in vitro may reflect a similar process in vivo, i.e., that one process contributing to development of atherosclerosis can be oxidation of lipoprotein, with loss of lipid‐protein affinity and accumulation of lipid products in (or on) cells of the vascular system.