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Triglyceride analysis by consecutive liquid‐liquid partition and gas‐liquid chromatography. Ephedra nevadensis seed fat
Author(s) -
Litchfield Carter
Publication year - 1968
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531736
Subject(s) - triglyceride , chromatography , lipidology , clinical chemistry , chemistry , gas chromatography , cholesterol , biochemistry
The triglyceride composition of Ephedra nevadensis seed fat, which contains 16 different fatty acids, has been analyzed by a combination of liquid‐liquid partition and gas‐liquid chromatography. Triglycerides were first separated by liquid‐liquid partition chromatography. The recovered fractions were then analyzed by gas‐liquid chromatography to determine the molecular weights of the triglycerides present. Consecutive separation by these two techniques resolved this complex seed fat into 30 different triglyceride groups. A method for preparative liquid‐liquid partition chromatography of triglycerides is described in detail. Highly unsaturated triglyceride mixtures are easily resolved on the basis of “partition number” by using a hexadecane/nitroethane partition system.