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Net increase of lipid fatty acid in ehrlich ascites carcinoma during growth
Author(s) -
Baker Nome,
Mermier Pierre,
Wilson Leon
Publication year - 1973
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531722
Subject(s) - clinical chemistry , fatty acid , lipidology , extracellular fluid , ascites , extracellular , ehrlich ascites carcinoma , chemistry , biochemistry , in vivo , biology , medicine , in vitro , microbiology and biotechnology
The net rate of total lipid fatty acid accumulation was measured in cells and extracellular fluid of Ehrlich ascites carcinomas grown in mice. Between 5 and 12 days after ip tumor inoculation, the tumor volume increased from 2.5 to 17.7 ml and the tumor cell volume increased from 1.5 to 3.9 ml. Total lipid fatty acids increased from 60 to 160 μmol during this same period. Thus the amount of fatty acid present increased at a rate of ca. 10 nmol/min for the tumor (cells and fluid). This rate is much smaller than the rate of fatty acid influx into Ehrlich ascites tumor cells in vivo. The major lipid fatty acids in both whole tumor and extracellular fluid were 16∶0, 18∶0, 18∶1 and 18∶2; no major changes in fatty acid composition were observed during growth.