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Preparation and characterization of mouse intestinal phospholipase
Author(s) -
Ottolenghi Athos
Publication year - 1973
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531718
Subject(s) - biochemistry , phospholipase , phospholipid , enzyme , biology , hemolysis , phospholipase c , phospholipase d , phospholipase a , mitochondrion , chemistry , phospholipase a2 , membrane , immunology
Phospholipase has been prepared in a stable, partially purified form from the small intestine of mice infected with the tapeworm Hymenolepis nana . The enzyme(s) attacks diacylphosphatides with liberation of free fatty acids and a corresponding decrease in phospholipid phosphorus, without accumulation of lysophosphatides. The reactivity with various substrates is strongly influenced by their physical state, the presence of other lipids, proteins or detergents. The phospholipids of some biomembranes (mitochondria, microsomes, red cell ghosts) are readily hydrolyzed under customary reaction conditions. The ensuing biochemical, morphological and functional alterations have been documented. In contrast, the diacylphospholipids of the cell membrane (intact erythrocytes, Pseudomonas aerunginosa ) are not accessible to phospholipase action unless some alteration of the integrity of the cell is induced by physical or chemical means (hemolysis, polymyxin). The enzyme is proposed as a tool for the investigation of biomembranes and as a model for the study of phospholipase activity.