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Lipase from egg of southern corn rootworm
Author(s) -
Krysan James L.,
Guss Paul L.
Publication year - 1973
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531710
Subject(s) - tributyrin , triolein , triacetin , lipase , chemistry , hydrolysis , oleic acid , food science , glyceride , glycerol , chromatography , triacylglycerol lipase , esterase , emulsion , biochemistry , acetone , enzyme , fatty acid
The lipolytic activity in homogenates and aqueous extracts of acetone powders of eggs of the southern corn rootworm ( Diabrotica undecimpunctata howardi Barber) was studied. The general properties were determined using as substrate olive oil in an emulsion stabilized by gum arabic. Bovine serum albumin or Triton X‐100 were required in the assay system; they protected the enzyme from spontaneous denaturation. The lipase had activity optima at pH 7 and at 45 C. The preparation was inactive towards triacetin, and activity increased in the series tripropionin